Horse Serum, Sterile, Donor Herd, Heat Inactivated
Blood is drawn aseptically by closed-system collection in a dedicated bleeding lab from a donor herd kept under qualified professional observation. It is refrigerated within 8 hours and processed within 48 hours. The serum is thawed, 0.22 µm filtered, bottled and stored at -20°C, then thawed again, heat-inactivated at 56°C for 30 minutes and returned to -20°C.
Specifications
Physical State:
Frozen Liquid
Storage:
-20°C or below
Release criteria
Protein Concentration:
≥ 6.0 g/dL
Hemoglobin:
≤ 10.00 mg/dL
pH:
6.5 – 8.5
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Species: | Horse |
|---|---|
| Product Types: | Serum |
| Donor Sex: | Mixed |
| Processing Method: | Heat Inactivated |
| Sterility: | Sterile |
| Physical State: | Frozen Liquid |
| Protein Concentration: | ≥ 6.0 g/dL |
| Application Notes: | Reportedly useful in immunohistochemistry assays and western blot; as diluent, blocking agent, cell culture/ growth supplement and source of serum proteins. |
| Testing: | Hemolysis Total Protein pH reported Sterility: Passed, 14 day sterility testing |
| Storage Conditions: | -20°C or below |
| Shipping Conditions: | Dry ice |
| Packaging: | Poly bottle |
| Expiration: | 5 years from date of manufacture when stored frozen at -20°C or below; once thawed, hold at 2 – 8°C and use within 4 weeks |
Product Applications
- Western blot
- Immunohistochemistry
- Blocking protein
- Assay diluent & negative matrix
- Cell culture
Documents & Datasheets
Datasheet
Safety Datasheet
Certificate of Analysis
| Lot | Issued | |
|---|---|---|
| 05847 | Mar 7, 2025 | Download |
| 29036 | Oct 30, 2014 | Download |
| 21236 | Sep 3, 2014 | Download |
| 15736 | Jun 12, 2014 | Download |
| 10136 | Apr 29, 2014 | Download |
| 04236 | Feb 28, 2014 | Download |
| 27535 | Oct 23, 2013 | Download |
| 22735 | Aug 22, 2013 | Download |
| 22234 | Aug 16, 2012 | Download |
| 13134 | Jun 20, 2012 | Download |
Frequently Asked Questions
How should I thaw frozen serum or plasma?
Put the bottle in a refrigerator at 2-8°C overnight, or in a 37°C water bath with the water kept below the cap, and swirl it gently as it thaws. Take it out as soon as the last ice has melted; never thaw above 37°C, on the bench or in a microwave. Then mix, aliquot what you will not use right away and return the aliquots to the label temperature. The full walkthrough with diagrams: how to thaw frozen serum.
Can I refreeze serum after it has thawed?
Aliquots can go back to the freezer once; the bulk bottle should not. Every freeze-thaw cycle concentrates salts, shifts pH and stresses the proteins, and complement activity drops with each one. Split the bottle into single-use tubes on the first thaw so each portion is only ever thawed once, or hold the thawed bottle at 2-8°C and use it within the window on the product page. See how to thaw frozen serum.
My thawed serum is cloudy or has particles in it. Is it still good?
Usually yes. Some haze or flocculent material after thawing is cryoprecipitate (fibrin, lipoprotein and calcium salts that came out of solution while frozen), and it does not change how the serum performs. Warm the bottle briefly to 37°C and swirl to redissolve most of it, or filter through a 0.45 µm filter if you need a clear product. A strong odor, a color change, or turbidity that keeps increasing at room temperature is contamination, not cryoprecipitate: do not use the bottle; contact us with the lot number. More in how to thaw frozen serum.
What is heat-inactivated serum and when should I use it?
Heat-inactivated serum has been held at 56°C for 30 minutes to destroy complement activity. Use it when you need the other serum components without functional complement, for example as a cell culture supplement or an assay matrix where active complement could lyse cells or cause non-specific killing.
Should I buy the sterile or the non-sterile version?
Sterile sera and plasmas are sterile-filtered, for cell culture and any other application that cannot tolerate bioburden. Non-sterile products are collected just as carefully but skip the filtration step, so they cost less and are the usual pick where sterility is not required, such as diagnostic reagent manufacturing.
How are your sterile-filtered products made?
Sterile-filtered products are manufactured using a combination of pre-filtration steps optimized for each product and final filtration using 0.2 micron filters.
Show 3 more questions
What's the maximum lot size of animal blood products I can order?
Our typical animal blood product lot sizes range from 50 L to 200 L.
Are blood products aseptically collected?
Yes, depending on the species and collection environment, aseptic collection techniques are practiced whenever possible. In animal collection facilities where aseptic technique may not be feasible, materials are collected as carefully as possible by highly trained technicians. Animal blood products are collected in an abattoir environment.
Do you have processes in place for the proper segregation of animal-based products obtained from different species?
Yes. We segregate animal-based products by species in line with Regulation (EC) 1069/2009 and Regulation (EU) 142/2011, and USDA APHIS inspects our facility for compliance.
Product References
- Ionotropin is the mammalian digoxin-like material (DLM). It is a phosphocholine ester of a steroid with 23 carbon atoms
Chasalow, F. & Pierce-Cohen, L. Ionotropin is the mammalian digoxin-like material (DLM). It is a phosphocholine ester of a steroid with 23 carbon atoms. Steroids 136, 63–75 (2018).
Alternate Names
Equine Serum Sterile Donor Herd Heat Inactivated
