Chicken Anti-Vimentin

Chicken polyclonal antibody to human, mouse and rat Vimentin, supplied as total IgY fraction.

Chicken Anti-Vimentin
Chicken Anti-Vimentin
Immunostaining of tumors and precursor PanIN lesions from 4 week old LSL-KrasG12D; Cdkn2alox/lox; p48Cre (KIC) mice that were treated with 2G8 and/or gemcitabine, Gem. (Anti-Vimentin, green; Anti-E-cadherin, red). Photo courtesy of Rolf Brekken, University of Texas Southwestern. CC-BY-4.0
Immunostaining of tumors and precursor PanIN lesions from 4 week old LSL-KrasG12D; Cdkn2alox/lox; p48Cre (KIC) mice that were treated with 2G8 and/or gemcitabine, Gem. (Anti-Vimentin, green; Anti-E-cadherin, red). Photo courtesy of Rolf Brekken, University of Texas Southwestern. CC-BY-4.0
Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~50 kDa Vimentin protein.
Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~50 kDa Vimentin protein.
Immunostaining of HeLa cells labelled with anti-vimentin antibody 1:10,000, green), anti-actin (red), and DAPI (blue) labelling of nuclear DNA. The vimentin antibody labels the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Immunostaining of HeLa cells labelled with anti-vimentin antibody 1:10,000, green), anti-actin (red), and DAPI (blue) labelling of nuclear DNA. The vimentin antibody labels the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Immunostaining of mixed neuron/glial cultures stained with anti-Vimentin antibody 1:500, green) and anti-GFAP antibody 1:1000, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
Immunostaining of mixed neuron/glial cultures stained with anti-Vimentin antibody 1:500, green) and anti-GFAP antibody 1:1000, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody red, 1:500) and anti-vimentin antibody green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody red, 1:500) and anti-vimentin antibody green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin red, 1:500) and anti-vimentin green, 1:10,000). The blue is Hoechst staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.
Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin red, 1:500) and anti-vimentin green, 1:10,000). The blue is Hoechst staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.
Immunolabeling of HeLa cells stained with anti-nuclear pore complex antibody red, 1:100) and rabbit anti-vimentin antibody green, 1:10,000). The blue is DAPI staining nuclear DNA. The anti-nuclear pore complex antibody reveals strong granular labeling of the nuclei corresponding to the protein. The anti-vimentin antibody specifically labels intermediate filaments.
Immunolabeling of HeLa cells stained with anti-nuclear pore complex antibody red, 1:100) and rabbit anti-vimentin antibody green, 1:10,000). The blue is DAPI staining nuclear DNA. The anti-nuclear pore complex antibody reveals strong granular labeling of the nuclei corresponding to the protein. The anti-vimentin antibody specifically labels intermediate filaments.
Immunostaining of HeLa cells stained with anti-nuclear pore complex antibody green, 1:100) and rabbit anti-vimentin antibody red, 1:500).
Immunostaining of HeLa cells stained with anti-nuclear pore complex antibody green, 1:100) and rabbit anti-vimentin antibody red, 1:500).
Immunoflourescence of HeLa cells stained with Anti-Park7 green, 1:1000) showing strong cytoplasmic staining and Anti-Vimentin red, 1:10,000) labeling cytoplasmic intermediate filaments. The blue is nuclear staining.
Immunoflourescence of HeLa cells stained with Anti-Park7 green, 1:1000) showing strong cytoplasmic staining and Anti-Vimentin red, 1:10,000) labeling cytoplasmic intermediate filaments. The blue is nuclear staining.
Immunoflourescence of HeLa cells labeled with anti-SAP49 red, 1:1000) and anti-vimentin green, 1:10,000). The blue is DAPI staining nuclear DNA.
Immunoflourescence of HeLa cells labeled with anti-SAP49 red, 1:1000) and anti-vimentin green, 1:10,000). The blue is DAPI staining nuclear DNA.
Immunofluorescence of HeLa cells showing strong granular nuclear staining of SAP49 (red, 1:1000) and vimentin green, 1:10,000). The blue is DAPI staining nuclear DNA.
Immunofluorescence of HeLa cells showing strong granular nuclear staining of SAP49 (red, 1:1000) and vimentin green, 1:10,000). The blue is DAPI staining nuclear DNA.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: ACKVIMIYV1

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Product Overview

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Recombinant human vimentin purified from E. coli.
Immunogen Species: Human
Species Reactivity: Bovine, Human, Mouse, Rat
Gene Name: VIM
Molecular Weight: 50 kDa
RRID: AB_2492283
Purification: Total IgY fraction
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes ~50 kDa Vimentin protein.
Application Notes: Recommended starting dilutions: WB 1:1000; IHC 1:500 – 1:5000; ICC 1:500 – 1:10,000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
Documents & Datasheets

Certificate of Analysis