Chicken Anti-S100B

Chicken polyclonal antibody to human, mouse and rat S100B, supplied as affinity-purified IgY.

DAPI nuclear stain (blue) shows nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-S100B signal and anti-flag in transfected cells.
DAPI nuclear stain (blue) shows nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-S100B signal and anti-flag in transfected cells.
Sagittal section of formalin fixed, paraffin-embedded rat brain showing staining of S100B. Images at right show higher magnification of indicated areas of interest (cerebellum, hippocampus, and cortex).
Sagittal section of formalin fixed, paraffin-embedded rat brain showing staining of S100B. Images at right show higher magnification of indicated areas of interest (cerebellum, hippocampus, and cortex).
Chicken Anti-S100B
Chicken Anti-S100B
Example of Schwann cells at the neuromuscular junction (NMJ) in 4% paraformaldehyde fixed mouse skeletal muscle tissue labeled with S100 primary (1:400). Nicotinic acetylcholine receptors (red) indicate the postsynaptic region of the NMJ. S100-stained terminal Schwann cells (green) lie over the receptors, while the end of the myelin sheath produced by a myelinating Schwann cell (green) is visible to the right. Image kindly provided by Robert Hastings, Brown University.
Example of Schwann cells at the neuromuscular junction (NMJ) in 4% paraformaldehyde fixed mouse skeletal muscle tissue labeled with S100 primary (1:400). Nicotinic acetylcholine receptors (red) indicate the postsynaptic region of the NMJ. S100-stained terminal Schwann cells (green) lie over the receptors, while the end of the myelin sheath produced by a myelinating Schwann cell (green) is visible to the right. Image kindly provided by Robert Hastings, Brown University.

Specifications

Physical State:
Liquid
Storage:
2 – 8°C
Buffer:
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative

Release criteria

Protein Concentration:
0.2 mg/mL
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Product code: ACKS1CIY

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Unit priceFrom $144.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: His tagged full length protein
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: S100B
Molecular Weight: 14 kDa
RRID: AB_2943661
Purification: Affinity-Purified IgY
Physical State: Liquid
Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative
Protein Concentration: 0.2 mg/mL
Specificity: No cross-reactivity reported.
Application Notes: WB: 1:500-1:1000 IHC: 1:500-1:1000 ICC: 1:100-1:500
Testing: Each new lot of this antibody is tested in WB to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
Storage Conditions: 2 – 8°C
Shipping Conditions: Dry ice
Expiration: Stable for 12 months from receipt.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis