Chicken Anti-P-Zero Myelin Protein (PZO)

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (NP_032649) and human (NP_000521) gene products.

Chicken Anti-P-Zero Myelin Protein (PZO)
Chicken Anti-P-Zero Myelin Protein (PZO)
In the image (a tissue section through an adult sciatic nerve), Po (green staining) can be seen in the myelin and Schwann cell processes surrounding the nodes of Ranvier. In this photomicrograph, rabbit antibodies against LAMP (lysozome-associated membrane glycoprotein) (red staining) serves as the counterstain, and DAPI (blue staining) allows visualization of nuclei.
In the image (a tissue section through an adult sciatic nerve), Po (green staining) can be seen in the myelin and Schwann cell processes surrounding the nodes of Ranvier. In this photomicrograph, rabbit antibodies against LAMP (lysozome-associated membrane glycoprotein) (red staining) serves as the counterstain, and DAPI (blue staining) allows visualization of nuclei.
Immunofluorescence of a section of injured (right, crushed) and normal (left) mouse sciatic nerve labeled with Anti-P-Zero Myelin Protein (1:1000, red), colabeled with Anti-Neurofilament NF-L (green), and DAPI labeling nuclei. Scale bar, 50 μm; Magnification, 20×. Image kindly provided by Prem Kumar, Department of Orthopedics and Rehabilitation, Penn State University College of Medicine, John C. Elfar lab.
Immunofluorescence of a section of injured (right, crushed) and normal (left) mouse sciatic nerve labeled with Anti-P-Zero Myelin Protein (1:1000, red), colabeled with Anti-Neurofilament NF-L (green), and DAPI labeling nuclei. Scale bar, 50 μm; Magnification, 20×. Image kindly provided by Prem Kumar, Department of Orthopedics and Rehabilitation, Penn State University College of Medicine, John C. Elfar lab.
Remyelinated lesion of index case stained for PLP (green) and Pzero (red), showing that the myelin is distinctly from oligodendrocyte or Schwann cell origin, respectively. Image courtesy of Bryan Bollman, Washington University in St. Louis.
Remyelinated lesion of index case stained for PLP (green) and Pzero (red), showing that the myelin is distinctly from oligodendrocyte or Schwann cell origin, respectively. Image courtesy of Bryan Bollman, Washington University in St. Louis.
P-zero (brown staining) in all of the myelinating Schwann cells of a tissue section of an adult sciatic nerve.
P-zero (brown staining) in all of the myelinating Schwann cells of a tissue section of an adult sciatic nerve.
Characterization of mouse vagus nerve derived Schwann cells (VNSCs). The identity and purity of SCs were confirmed using IF staining of S100 (pink), p75NTR (green), and Mpz (1:1000, red) under a fluorescent microscope (ZEISS Apotome 2). The purity of the cultured SCs (99%) was analyzed by double positive staining of DAPI with S100/p75NTR/Mpz markers. Each image represents 3 images from 3 independent experiments. Scale bar: 50 μm, n = 3. Image from publication CC-BY-4.0. PMID: 37127673
Characterization of mouse vagus nerve derived Schwann cells (VNSCs). The identity and purity of SCs were confirmed using IF staining of S100 (pink), p75NTR (green), and Mpz (1:1000, red) under a fluorescent microscope (ZEISS Apotome 2). The purity of the cultured SCs (99%) was analyzed by double positive staining of DAPI with S100/p75NTR/Mpz markers. Each image represents 3 images from 3 independent experiments. Scale bar: 50 μm, n = 3. Image from publication CC-BY-4.0. PMID: 37127673
Representative immunofluorescence images of mouse sciatic nerve teased fibers at postnatal day 30. Fibers were stained for P0 (green), endoplasmic reticulum (ER) (KDEL, red), and DAPI (blue). Image from publication CC-BY-4.0. PMID: 36350884
Representative immunofluorescence images of mouse sciatic nerve teased fibers at postnatal day 30. Fibers were stained for P0 (green), endoplasmic reticulum (ER) (KDEL, red), and DAPI (blue). Image from publication CC-BY-4.0. PMID: 36350884
Representative immunofluorescence images of mouse sciatic nerve cross sections at 2 months of age. Cross sections were stained for P0 (green), endoplasmic reticulum (ER) (KDEL, red), and DAPI (blue). Image from publication CC-BY-4.0. PMID: 36350884
Representative immunofluorescence images of mouse sciatic nerve cross sections at 2 months of age. Cross sections were stained for P0 (green), endoplasmic reticulum (ER) (KDEL, red), and DAPI (blue). Image from publication CC-BY-4.0. PMID: 36350884
Western blot analysis of P0 in sciatic nerve lysate from wild-type (WT), MpzT124M/+ (TM/+), and MpzT124M/T124M (TM/TM) mice treated with (cut) or without (uncut) EndoH (A) and PNGase F (B). Image from publication CC-BY-4.0. PMID: 36350884
Western blot analysis of P0 in sciatic nerve lysate from wild-type (WT), MpzT124M/+ (TM/+), and MpzT124M/T124M (TM/TM) mice treated with (cut) or without (uncut) EndoH (A) and PNGase F (B). Image from publication CC-BY-4.0. PMID: 36350884
In mature myelinating cultures, SCs CASPR 1:500; white) can be detected in the characteristic staining pattern marking paranodes. Scale bar 5 μm. Paranodal CASPR co-localised on NFL-labelled axons (magenta). Scale bar: 5 μm. When colabelling with MPZ (1:500; green), CASPR is localised to paranodal loops adjacent to a node of Ranvier. Scale bar: 10 μm. Image from publication CC-BY-4.0. PMID:37642648
In mature myelinating cultures, SCs CASPR 1:500; white) can be detected in the characteristic staining pattern marking paranodes. Scale bar 5 μm. Paranodal CASPR co-localised on NFL-labelled axons (magenta). Scale bar: 5 μm. When colabelling with MPZ (1:500; green), CASPR is localised to paranodal loops adjacent to a node of Ranvier. Scale bar: 10 μm. Image from publication CC-BY-4.0. PMID:37642648
Confocal image of MPZ (1:500; green)-labelled myelinating mouse Schwann cells (SCs). Image from publication CC-BY-4.0. PMID:37642648
Confocal image of MPZ (1:500; green)-labelled myelinating mouse Schwann cells (SCs). Image from publication CC-BY-4.0. PMID:37642648
Western blot analysis of PMP2 and MPZ (1:5000) in control, Bace1−/−, NRG1t3OE, Bace1−/−; NRG1t3OE, NRG1t3ΔC (Constitutively active NRG1 type III) and Bace1−/−; NRG1t3ΔC at 60 days of age (C,D). Tubulin (TUB) was used for the endogenous control for the Western blots. Image from publication CC-BY-4.0. PMID:38311982
Western blot analysis of PMP2 and MPZ (1:5000) in control, Bace1−/−, NRG1t3OE, Bace1−/−; NRG1t3OE, NRG1t3ΔC (Constitutively active NRG1 type III) and Bace1−/−; NRG1t3ΔC at 60 days of age (C,D). Tubulin (TUB) was used for the endogenous control for the Western blots. Image from publication CC-BY-4.0. PMID:38311982

Specifications

Physical State:
Liquid
Storage:
2 – 8°C
Buffer:
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative

Release criteria

Protein Concentration:
200 µg/mL
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Product code: ACKPZ1IY

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Product Overview

Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Synthetic peptides
Immunogen Species: Human
Species Reactivity: Human, Mouse, Pig, Rat
Gene Name: MPZ
Molecular Weight: 27 kDa
RRID: AB_2313561
Purification: Affinity-Purified IgY
Physical State: Liquid
Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative
Protein Concentration: 200 µg/mL
Application Notes: Recommended starting dilutions: WB 1:10000 – 1:20000; IHC 1:2000 – 1:5000; ICC 1:2000 – 1:5000.
Testing: Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Storage Conditions: 2 – 8°C
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles.
Shipping Conditions: Dry ice
Expiration: Stable for 12 months from receipt.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis