Chicken Anti-NFM (Neurofilament Medium Chain)
NFM protein was purified from bovine spinal cords following the method of Delacourte et al.
Specifications
Physical State:
Liquid
Storage:
2 – 8°C
Buffer:
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic, sodium azide (0.02%, w/v) added as a preservative
Release criteria
Protein Concentration:
2 mg/mL
Lead time on this item is currently: 2-4 Weeks
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Product Overview
["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion". Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. After several boosts, hens were co-boosted with recombinant NFM protein expressed in bacteria. These antibodies have been validated with human, mouse and rat tissues.
| Host Species: | Chicken |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgY |
| Immunogen: | Recombinant construct containing the C-terminal region of the human sequence expressed in Escherichia coli. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | NEFM NEF3 NFM |
| Molecular Weight: | 150 kDa |
| RRID: | AB_2313554 |
| Purification: | IgY Fraction |
| Physical State: | Liquid |
| Buffer: | Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic, sodium azide (0.02%, w/v) added as a preservative |
| Protein Concentration: | 2 mg/mL |
| Application Notes: | Recommended starting dilutions: WB 1:1000 – 1:2000; IHC 1:1000 – 1:2000; ICC 1:1000 – 1:2000. |
| Testing: | Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent. |
| Storage Conditions: | 2 – 8°C |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt. |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Neuroscience research