Chicken Anti-NFH (Neurofilament Heavy Chain)

NFH protein was purified from bovine spinal cords following the method of Delacourte et al.

Chicken Anti-NFH (Neurofilament Heavy Chain)
Chicken Anti-NFH (Neurofilament Heavy Chain)
Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution)
Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution)
Confocal micrographs of DRG cryosections from WT mouse double-labeled with antibodies against caldendrin (green) and NF200 (1:1000, purple) or peripherin (1:1000, purple). Arrows and arrowheads indicate cells in which caldendrin is or is not, respectively, co-localized with NF200 or peripherin. Results are representative of at least 3 independent experiments. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Confocal micrographs of DRG cryosections from WT mouse double-labeled with antibodies against caldendrin (green) and NF200 (1:1000, purple) or peripherin (1:1000, purple). Arrows and arrowheads indicate cells in which caldendrin is or is not, respectively, co-localized with NF200 or peripherin. Results are representative of at least 3 independent experiments. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Confocal micrographs of caldendrin KO cultures infected with AAV-mRuby (top) or AAV-mRuby-caldendrin (bottom) showing immunolabeling for NF200 (1:1000, left) and mRuby (right). NF200-positive DRGNs with and without mRuby fluorescence are indicated by arrows and arrowheads, respectively. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Confocal micrographs of caldendrin KO cultures infected with AAV-mRuby (top) or AAV-mRuby-caldendrin (bottom) showing immunolabeling for NF200 (1:1000, left) and mRuby (right). NF200-positive DRGNs with and without mRuby fluorescence are indicated by arrows and arrowheads, respectively. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334
Representative images of sections of a rat G muscle 7, 14, 21, and 60 dpi, immunostained against NFH (1:200; green), α-btx (red), and VAChT (blue), respectively, to label axons, neuromuscular junctions, and the synaptic vesicle-filled motor end plate. Larger numbers of reinnervated NMJs were observed with increased time after injury, with almost complete innervation at 60 dpi. The nicotinic receptor field expands after early denervation. Image from publication CC-BY-4.0. PMID: 36759186
Representative images of sections of a rat G muscle 7, 14, 21, and 60 dpi, immunostained against NFH (1:200; green), α-btx (red), and VAChT (blue), respectively, to label axons, neuromuscular junctions, and the synaptic vesicle-filled motor end plate. Larger numbers of reinnervated NMJs were observed with increased time after injury, with almost complete innervation at 60 dpi. The nicotinic receptor field expands after early denervation. Image from publication CC-BY-4.0. PMID: 36759186
Example images of back hairy skin wholemount staining of NFH (1:500) and S100B in control and mutant mice. Overall innervation and lanceolate ending morphologyImage from publication CC-BY-4.0. PMID:37607547
Example images of back hairy skin wholemount staining of NFH (1:500) and S100B in control and mutant mice. Overall innervation and lanceolate ending morphologyImage from publication CC-BY-4.0. PMID:37607547
Forelimb pedal pad sections of control Npy2r-GFP glabrous skin (top) and Prx1Cre; Lmx1bfl/fl; Npy2r-GFP double glabrous mutant, ectopic glabrous skin (bottom). The sections are stained using S100, GFP, and NFH (1:500). Red arrows point to the GFP- axon (presumably Ret+).Image from publication CC-BY-4.0. PMID:37607547
Forelimb pedal pad sections of control Npy2r-GFP glabrous skin (top) and Prx1Cre; Lmx1bfl/fl; Npy2r-GFP double glabrous mutant, ectopic glabrous skin (bottom). The sections are stained using S100, GFP, and NFH (1:500). Red arrows point to the GFP- axon (presumably Ret+).Image from publication CC-BY-4.0. PMID:37607547
Sections of P21 control and double glabrous forepaws with NFH (1:500), S100, and DAPI. Control ventral and dorsal skin regions (top two rows) have Meissner corpuscles on the ventral side and lanceolate endings on the dorsal side. Double glabrous mutant ventral and dorsal skin (bottom two rows) is predominantly glabrous, and these mutants contain Meissner corpuscles on both the ventral and dorsal sides of the paw. Image from publication CC-BY-4.0. PMID:37607547
Sections of P21 control and double glabrous forepaws with NFH (1:500), S100, and DAPI. Control ventral and dorsal skin regions (top two rows) have Meissner corpuscles on the ventral side and lanceolate endings on the dorsal side. Double glabrous mutant ventral and dorsal skin (bottom two rows) is predominantly glabrous, and these mutants contain Meissner corpuscles on both the ventral and dorsal sides of the paw. Image from publication CC-BY-4.0. PMID:37607547
(A and B) Example images of TrkB+ endings (TrkBGFP or TrkBCreER; AvilFlp; RosaLSL-FSF-Tdtomato) in paw glabrous skin (A) and paw hairy skin (B) at E15.5, P5, and P50. The sections were stained with S100, NFH (1:500) and GFP or tdTomato. The white dotted lines represent the dermal-epidermal junction in glabrous skin and the hair follicle outline in hairy skin. Image from publication CC-BY-4.0. PMID:37607547
(A and B) Example images of TrkB+ endings (TrkBGFP or TrkBCreER; AvilFlp; RosaLSL-FSF-Tdtomato) in paw glabrous skin (A) and paw hairy skin (B) at E15.5, P5, and P50. The sections were stained with S100, NFH (1:500) and GFP or tdTomato. The white dotted lines represent the dermal-epidermal junction in glabrous skin and the hair follicle outline in hairy skin. Image from publication CC-BY-4.0. PMID:37607547
Representative images of mouse spinal cord throughout the rostral-caudal extent of injury 42 days after spinal cord injury (SCI). Sections are stained for myelin (blue, erichrome cyanine) and axons (brown, neurofilament, 1:1500). Frank lesion areas are highlighted in red. Spared tissue is evident by both blue and brown staining. Image from publication CC-BY-4.0. PMID:37350129
Representative images of mouse spinal cord throughout the rostral-caudal extent of injury 42 days after spinal cord injury (SCI). Sections are stained for myelin (blue, erichrome cyanine) and axons (brown, neurofilament, 1:1500). Frank lesion areas are highlighted in red. Spared tissue is evident by both blue and brown staining. Image from publication CC-BY-4.0. PMID:37350129
NFH (axonal tracts,) and MBP (myelin) immunostaining of the corpus callosum sections. Compared with WT (C57BL/6 wildtype) and mTrappc9+/+, the corpus callosum (highlighted with white dotted lines) of mTrappc9m/m mice was significantly thinner. Image from publication CC-BY-4.0. PMID:37416774
NFH (axonal tracts,) and MBP (myelin) immunostaining of the corpus callosum sections. Compared with WT (C57BL/6 wildtype) and mTrappc9+/+, the corpus callosum (highlighted with white dotted lines) of mTrappc9m/m mice was significantly thinner. Image from publication CC-BY-4.0. PMID:37416774
Western blot analysis of MBP and NFH protein expression in cerebral cortex normalized with GAPDH. Both showed a significant decrease in mTrappc9 m/m mice. Image from publication CC-BY-4.0. PMID:37416774
Western blot analysis of MBP and NFH protein expression in cerebral cortex normalized with GAPDH. Both showed a significant decrease in mTrappc9 m/m mice. Image from publication CC-BY-4.0. PMID:37416774

Specifications

Physical State:
Liquid
Storage:
2 – 8°C
Buffer:
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic, sodium azide (0.02%, w/v) added as a preservative

Release criteria

Protein Concentration:
1 mg/mL
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Product code: ACKNFHIYHV-200UL

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Unit price$301.00
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Product Overview

["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion”. Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFH protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been found to cross-react with NFH protein from a variety of species, including human, mouse and rat.

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Native NF-H purified from bovine spinal cord.
Immunogen Species: Bovine
Species Reactivity: Human, Mouse, Rat
Gene Name: NEFH KIAA0845 NFH
Molecular Weight: 200-220 kDa
RRID: AB_2313552
Purification: IgY Fraction
Physical State: Liquid
Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic, sodium azide (0.02%, w/v) added as a preservative
Protein Concentration: 1 mg/mL
Application Notes: Recommended starting dilutions: WB 1:5000 – 1:10000; IHC 1:2000 – 1:5000; ICC 1:2000 – 1:5000.
Testing: Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:50000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Storage Conditions: 2 – 8°C
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles.
Shipping Conditions: Dry ice
Expiration: Stable for 12 months from receipt.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis