Chicken Anti-Neurofilament NF-M

Chicken polyclonal antibody to bovine, chicken, human, mouse and rat Neurofilament NF-M, supplied as total IgY fraction.

Chicken Anti-Neurofilament NF-M
Chicken Anti-Neurofilament NF-M
Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.
Immunofluorescence of a section of rat cerebellum labeled with anti-Neurofilament-M 1:1000, red) and anti-CNP (green). The anti-NFM labels the network of axons of basket neurons and other neurons. The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat cerebellum labeled with anti-Neurofilament-M 1:1000, red) and anti-CNP (green). The anti-NFM labels the network of axons of basket neurons and other neurons. The blue is DAPI staining of nuclear DNA.
Immunostaining of cultured rat neurons and glia stained with anti-NFM antibody red, 1:1000) and DNA is stained blue.
Immunostaining of cultured rat neurons and glia stained with anti-NFM antibody red, 1:1000) and DNA is stained blue.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: ACKNFMIY

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Unit priceFrom $154.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Preparation containing the extreme C-terminal region of rat NF-M, expressed in and purified from E. coli.
Immunogen Species: Rat
Species Reactivity: Bovine, Chicken, Human, Mouse, Rat
Gene Name: NEFM
Molecular Weight: 145 kDa
RRID: AB_2492164
Purification: Total IgY fraction
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~145 kDa Neurofilament M protein.
Application Notes: Recommended starting dilutions: WB 1:5000; WB Brain 1:5000; IHC 1:1000; ICC 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis