Chicken Anti-Neurofilament NF-L

Chicken polyclonal antibody to bovine, chicken, human, mouse and rat Neurofilament NF-L, supplied as total IgY fraction.

Chicken Anti-Neurofilament NF-L
Chicken Anti-Neurofilament NF-L
Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L 1:2000, green) and labeling of FOX3/NeuN 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L 1:2000, green) and labeling of FOX3/NeuN 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFL antibody red, 1:5000). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites, and perikarya as shown above.
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFL antibody red, 1:5000). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites, and perikarya as shown above.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: ACKNEFIY

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Unit priceFrom $154.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Preparation of bovine spinal cord NF-L.
Immunogen Species: Bovine
Species Reactivity: Bovine, Chicken, Human, Mouse, Rat
Gene Name: NEFL
Molecular Weight: 68 kDa
RRID: AB_2492162
Purification: Total IgY fraction
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~68 kDa Neurofilament L. Works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.
Application Notes: Recommended starting dilutions: WB 1:10000; WB Brain 1:10000; IHC 1:2000; ICC 1:2000 – 1:5000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis