Chicken Anti-Neurofilament Medium Polypeptide (NF-M)
Affinity purified chicken polyclonal antibody raised against Neurofilament Medium Polypeptide (NF-M).
Specifications
Physical State:
Liquid
Storage:
-20°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized IgY preparation, with sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
Isotype IgY.
| Host Species: | Chicken |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgY |
| Immunogen: | This antibody has been made against a recombinant fusion protein containing the extreme C-terminal region of human NF-M (residues 708-877) expressed in and purified from E. coli. |
| Immunogen Species: | Human |
| Species Reactivity: | Bovine, Chicken, Horse, Human, Mouse, Pig, Rat |
| Molecular Weight: | ~145 kDa for rodent and ~160 kDa for human and cow |
| Purification: | IgY preparation |
| Physical State: | Liquid |
| Buffer: | Lyophilized IgY preparation, with sodium azide |
| Specificity: | Species cross-reactivity includes human, rat, mouse, cow, pig, horse and chicken. Band appears at ~145 kDa for rodent and ~160 kDa for human and cow in WB. Predicted to react with other mammalian tissues due to sequence homology. |
| Application Notes: | Recommended starting dilutions: WB 1:2000 – 1:5000; IHC 1:2000; ICC 1:2000. |
| Storage Conditions: | -20°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Neuroscience research