Chicken Anti-Neurofilament Light Polypeptide (NF-L), DG-Sensor
Affinity purified chicken polyclonal antibody raised against Neurofilament Light Polypeptide (NF-L).
Specifications
Physical State:
Liquid
Storage:
-20°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
Isotype IgY.
| Host Species: | Chicken |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgY |
| Immunogen: | Raised against a proprietary recombinant construct containing residues of human NF-L expressed in and purified from E Coli. |
| Immunogen Species: | Human |
| Species Reactivity: | Bovine, Human, Mouse, Pig, Rat |
| Purification: | IgG |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide |
| Specificity: | Species cross-reactivity includes human, rat, mouse, cow, and pig. These are antibodies which recognize epitopes in a small segment of the neurofilament NF-L subunit which are normally not accessible to antibodies but which became available on degeneration in natural or native conditions. Under reduced or proteinase treated tissues or Western blot, these antibodies are all specific for NF-L from a variety of species. |
| Application Notes: | Recommended starting dilutions: WB 1:1000 – 1:5000; IHC 1:1000 – 1:10000; ICC 1:1000 – 1:10000. |
| Storage Conditions: | -20°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Neuroscience research