Chicken Anti-Neurofilament Heavy Polypeptide, Phosphorylated, (pNF-H)
Affinity purified chicken polyclonal antibody raised against Neurofilament Heavy Polypeptide, Phosphorylated, (pNF-H).
Specifications
Physical State:
Liquid
Storage:
-20°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized IgY preparation, with sodium azide
Lead time on this item is currently: 2-4 Weeks
Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426
Product Overview
Isotype IgY.
| Host Species: | Chicken |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgY |
| Immunogen: | This antibody has been made against biochemically isolated NF-H purified from bovine spinal cord. |
| Immunogen Species: | Bovine |
| Species Reactivity: | Bovine, Canine, Horse, Human, Mouse, Pig, Rat |
| Molecular Weight: | 200 kDa |
| Purification: | IgY preparation |
| Physical State: | Liquid |
| Buffer: | Lyophilized IgY preparation, with sodium azide |
| Specificity: | Species cross-reactivity includes human, rat, mouse, cow, pig, horse and dog. This antibody reacts with phosphorylated NF-H and is seen as a band at approx 200 kDa in WB. Refer to publication by Shaw et al (2005) for the use of this antibody in an ELISA to detect NF-H. Predicted to react with other mammalian tissues due to sequence homology. |
| Application Notes: | Recommended starting dilutions: WB 1:20000 – 1:50000; IHC 1:20000; ICC 1:20000. |
| Storage Conditions: | -20°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- ELISA
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Neuroscience research