Chicken Anti-Myelin Basic Protein (MBP)

Chicken polyclonal antibody to bovine, human, mouse and rat Myelin Basic Protein (MBP), supplied as total IgY fraction.

Chicken Anti-Myelin Basic Protein (MBP)
Chicken Anti-Myelin Basic Protein (MBP)
Immunofluorescence of a section of rat cerebellum showing specific labeling of myelin basic protein 1:5000, green) in the oligodendrocytes and myelin sheathes around axons. The section is colabeled with Anti-NFL (red) labeling dendrites and axons of neuronal cells. The blue stains nuclear DNA.
Immunofluorescence of a section of rat cerebellum showing specific labeling of myelin basic protein 1:5000, green) in the oligodendrocytes and myelin sheathes around axons. The section is colabeled with Anti-NFL (red) labeling dendrites and axons of neuronal cells. The blue stains nuclear DNA.
Western blot of rat cortical lysate showing specific immunolabeling of the ~18 kDa and ~22 kDa MBP protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~18 kDa and ~22 kDa MBP protein.
Immunofluorescence of a perpendicular section from mouse spinal cord gray matter (upper left corner) and white matter (lower right corner) labeling myelin basic protein (magenta, 1:500) from oligodendrocytes and myelin sheaths. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
Immunofluorescence of a perpendicular section from mouse spinal cord gray matter (upper left corner) and white matter (lower right corner) labeling myelin basic protein (magenta, 1:500) from oligodendrocytes and myelin sheaths. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
Immunofluorescence of a cross-section from mouse corpus callosum labeling myelin basic protein (magenta, 1:500) in oligodendrocytes and myelin sheaths. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
Immunofluorescence of a cross-section from mouse corpus callosum labeling myelin basic protein (magenta, 1:500) in oligodendrocytes and myelin sheaths. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
Immunostaining of cultured neonatal rat neurons and glia stained with anti-myelin basic protein antibody red, 1:500). The blue stain is to identify nuclear DNA. Note the antibody stains the myelin basic protein located in oligodendrocyte and myelin sheathes around axons in the cells.
Immunostaining of cultured neonatal rat neurons and glia stained with anti-myelin basic protein antibody red, 1:500). The blue stain is to identify nuclear DNA. Note the antibody stains the myelin basic protein located in oligodendrocyte and myelin sheathes around axons in the cells.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: ACKMBPIY

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Unit priceFrom $154.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Three peptide sequences conserved in higher vertebrate MBP proteins.
Immunogen Species: Rat
Species Reactivity: Bovine, Human, Mouse, Rat
Gene Name: MBP
Molecular Weight: 18/22 kDa
RRID: AB_2492157
Purification: Total IgY fraction
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~18 kDa & ~22 kDa myelin basic protein.
Application Notes: Recommended starting dilutions: WB 1:5000; WB Brain 1:5000; IHC 1:500 – 1:5000; ICC 1:500 – 1:2000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis