Chicken Anti-Ki-67

Chicken polyclonal antibody to human, mouse and rat Ki-67, supplied as total IgY fraction.

Immunostaining of HeLa cells showing specific labeling of Ki-67 1:2000, red) present in cytoplasmic microtubules. Additional immunostaining done with β-tubulin in green and nuclear staining with DAPI (blue). During cell cycle Ki-67 protein is predominantly expressed in the nucleoli of cells during mitosis and interphase, and is not present during quiescence.
Immunostaining of HeLa cells showing specific labeling of Ki-67 1:2000, red) present in cytoplasmic microtubules. Additional immunostaining done with β-tubulin in green and nuclear staining with DAPI (blue). During cell cycle Ki-67 protein is predominantly expressed in the nucleoli of cells during mitosis and interphase, and is not present during quiescence.
Western blotting of HeLa cell lysate (10 µg/lane) with Ki-67 antibody at 2 µg/mL dilution and detected with anti-chicken HRP.
Western blotting of HeLa cell lysate (10 µg/lane) with Ki-67 antibody at 2 µg/mL dilution and detected with anti-chicken HRP.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS + 10 mM NaN3
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Product code: ACKK67IY-100UL

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Unit price$301.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Recombinant human ki-67 protein expressed in and purified from E. Coli.
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: MKI67
Molecular Weight: 345-395 kDa
RRID: AB_2941673
Purification: Total IgY fraction
Physical State: Liquid
Buffer: PBS + 10 mM NaN3
Specificity: Specific for endogenous levels of the ~345 & 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these 2 isoforms.
Application Notes: WB: 1:5000 IHC: 1:1000-1:5000 ICC: 1:1000-1:5000
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis