Chicken Anti-Growth Associated Protein 43 (GAP-43)
Affinity purified chicken polyclonal antibody raised against Growth Associated Protein 43 (GAP-43).
Specifications
Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized IgY preparation, with sodium azide
Lead time on this item is currently: 2-4 Weeks
Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426
Product Overview
Isotype IgY.
| Host Species: | Chicken |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgY |
| Immunogen: | C-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein. |
| Immunogen Species: | Rat |
| Species Reactivity: | Bovine, Human, Rat |
| Molecular Weight: | 43 kDa |
| Purification: | Concentrated purified IgY |
| Physical State: | Liquid |
| Buffer: | Lyophilized IgY preparation, with sodium azide |
| Specificity: | Reacts with a 43 kDa band by Western blot on bovine hippocampus lysate. It has also been used successfully for immunocytochemistry. Human, rat and bovine. It can be expected that it will work on other mammal tissues due to amino acid sequence similarity. |
| Application Notes: | Recommended starting dilutions: WB 1:1000 – 1:5000; ICC 1:500 – 1:1000. |
| Storage Conditions: | -20°C or -80°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Neuroscience research