Chicken Anti-Glutamic Acid Decarboxylase-67 (GAD 67)

Affinity purified chicken polyclonal antibody raised against Glutamic Acid Decarboxylase-67 (GAD 67).

Chicken Anti-Glutamic Acid Decarboxylase-67 (GAD 67)
Chicken Anti-Glutamic Acid Decarboxylase-67 (GAD 67)
Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD.1 (green, 1:1000 dilution) immunoreactivity. Phalloidin (F-actin) (red) staining. DAPI (blue) staining. Photomicrograph from Page Balich (Univ. Arizona).
Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD.1 (green, 1:1000 dilution) immunoreactivity. Phalloidin (F-actin) (red) staining. DAPI (blue) staining. Photomicrograph from Page Balich (Univ. Arizona).
Immunohistochemical staining of inhibitory neurons (red; 1:500) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde. The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain. Secondary antibody (Texas Red goat anti-chicken IgY, (1:500).
Immunohistochemical staining of inhibitory neurons (red; 1:500) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde. The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain. Secondary antibody (Texas Red goat anti-chicken IgY, (1:500).
Immunocytochemical staining of GAD-67 (red/yellow) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (1:500). MAP2 staining (green) used a rabbit antibody (1:500).
Immunocytochemical staining of GAD-67 (red/yellow) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (1:500). MAP2 staining (green) used a rabbit antibody (1:500).
Immunocytochemical staining of GAD-67 (red/yellow) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (1:500). MAP2 staining (green) used a rabbit antibody (1:500).
Immunocytochemical staining of GAD-67 (red/yellow) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (1:500). MAP2 staining (green) used a rabbit antibody (1:500).

Specifications

Physical State:
Liquid
Storage:
2 – 8°C
Buffer:
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative

Release criteria

Protein Concentration:
100 µg/mL
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Product code: ACKG67IY

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Product Overview

Isotype IgY.

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Synthetic peptide
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: GAD1 GAD GAD67
Molecular Weight: 67 kDa
RRID: AB_2313545
Purification: Affinity-Purified IgY
Physical State: Liquid
Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative
Protein Concentration: 100 µg/mL
Application Notes: Recommended starting dilutions: WB 1:1000 – 1:2000; IHC 1:500 – 1:1000; ICC 1:500 – 1:1000.
Testing: This antibody preparation was analyzed by immunohisto-chemistry (at a concentration of 1 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Storage Conditions: 2 – 8°C
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles.
Shipping Conditions: Dry ice
Expiration: Stable for 12 months from receipt.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis