Chicken Anti-GAP43

Chicken polyclonal antibody to bovine, horse, human, mouse and rat GAP43, supplied as total IgY fraction.

Chicken Anti-GAP43
Chicken Anti-GAP43
Western blot of rat cortical lysate showing specific immunolabeling of the ~43 kDa GAP43 protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~43 kDa GAP43 protein.
Immunolabeling of mixed neuron and glial cultures with anti-GAP43 1:2000, green), alpha II spectrin 1:1000, red) and nuclear staining with DAPI (blue). The anti-GAP43 labels protein expressed in the axonal membrane and synapses of neuronal cells. The Anti-αII-Spectrin labels the submembraneous cytoskeleton of axons and dendrites.
Immunolabeling of mixed neuron and glial cultures with anti-GAP43 1:2000, green), alpha II spectrin 1:1000, red) and nuclear staining with DAPI (blue). The anti-GAP43 labels protein expressed in the axonal membrane and synapses of neuronal cells. The Anti-αII-Spectrin labels the submembraneous cytoskeleton of axons and dendrites.
Immunofluorescence of rat neuron/glial culture labeling GAP43 protein 1:2000, green) expressed in the axonal membrane and synapses of neuronal cells. DAPI (blue) was used for nuclear staining.
Immunofluorescence of rat neuron/glial culture labeling GAP43 protein 1:2000, green) expressed in the axonal membrane and synapses of neuronal cells. DAPI (blue) was used for nuclear staining.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: ACKG43IY

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Unit priceFrom $154.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Synthetic peptide from the C-terminal region of rat GAP43, conjugated to keyhole limpet hemocyanin (KLH).
Immunogen Species: Rat
Species Reactivity: Human, Mouse, Rat
Gene Name: GAP43
Molecular Weight: 43 kDa
RRID: AB_2492122
Purification: Total IgY fraction
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~43 kDa GAP-43 protein.
Application Notes: Recommended starting dilutions: WB 1:2000; WB Brain 1:2000; ICC 1:500 – 1:2000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis