Chicken Anti-Alpha Internexin/ NF-66

Chicken polyclonal antibody to human, mouse and rat Alpha Internexin/ NF-66, supplied as total IgY fraction.

Chicken Anti-Alpha Internexin/ NF-66
Chicken Anti-Alpha Internexin/ NF-66
Immunofluorescence of a section of rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha-internexin 1:5000, red), labeling of the myelin sheaths around the axons with MBP 1:5000, green), and additional nuclear staining with DAPI (blue).
Immunofluorescence of a section of rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha-internexin 1:5000, red), labeling of the myelin sheaths around the axons with MBP 1:5000, green), and additional nuclear staining with DAPI (blue).
Immunofluorescence of cultured neurons and glia cells showing specific labeling of neuronal processes with Anti-alpha-internexin 1:500, red), and specific labeling of microglia with anti-coronin 1a (green).
Immunofluorescence of cultured neurons and glia cells showing specific labeling of neuronal processes with Anti-alpha-internexin 1:500, red), and specific labeling of microglia with anti-coronin 1a (green).
Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66 kDa alpha internexin protein.
Western blot of rat cortex lysate showing specific immunolabeling of the ~ 66 kDa alpha internexin protein.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: ACKINAIY

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Unit priceFrom $154.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Full-length recombinant rat alpha-internexin, expressed in E. coli and purified.
Immunogen Species: Rat
Species Reactivity: Bovine, Human, Mouse, Rat
Gene Name: INA
Molecular Weight: 66 kDa
RRID: AB_2492033
Purification: Total IgY fraction
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~66 kDa alpha Internexin protein. Minor bands at ~150 kDa are probably covalent dimers and bands at ~50 kDa represent alpha-internexin breakdown products.
Application Notes: Recommended starting dilutions: WB 1:5000; WB Brain 1:5000; IHC 1:1000 – 1:5000; ICC 1:500 – 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis