Pel-Freez Blog
Read more about the latest updates, how Pel-Freez products support research, and get helpful practical tips in our blog.
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IgG/IgM-Depleted Human Serum: Why and How Antibodies Are RemovedWhy IgG and IgM are removed from human serum, how immunoaffinity depletion works, and where antibody-depleted serum serves as an SBA/OPA complement source.
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Key Attributes of Complement Products for ResearchWhat to look for in a complement reagent: species source, hemolytic activity, stability and storage, with guidance for SBA, OPA and cytotoxicity assays.
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Complement Activity Explained: CH50 and Hemolytic TiterWhat CH50 (50% hemolytic complement titer) measures, how it is run with antibody-coated sheep RBCs, and how to use it when qualifying complement reagents.
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Key Attributes of Albumins: BSA and HSA OverviewStructure, function and laboratory uses of bovine serum albumin (Cohn Fraction V) and human serum albumin, and how to pick the right albumin grade.
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Acetone Powders: Preparation, Attributes and UsesWhat acetone powders are, how tissue is acetone-dehydrated, and where rabbit brain, thymus and muscle powders are used, from thromboplastin to enzymes.
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Using Acetone Powders for Antibody Pre-adsorptionHow tissue acetone powders remove cross-reactive antibodies before IHC, Western blot or ELISA, with a step-by-step pre-adsorption protocol.
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Why quality and traceability matter in coagulation reagents for IVDBiologically derived coagulation reagents vary, and that variability costs IVD developers validation runs, time and regulatory goodwill. What to look for in a reagent...
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Learn How to Improve Immunogenicity Assay Accuracy with Antibody-Depleted ComplementDonor antibodies in native human complement create background in SBA and OPKA. The data behind our liter-scale IgG/IgM/IgA depletion, what it preserves, and where...
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Expanding luminescence-based SBA to 7 Shigella serotypes with Pel-Freez baby rabbit complementGSK Vaccines Institute for Global Health researchers extended a luminescence-based SBA to seven Shigella flexneri serotypes and found no difference between baby rabbit complement...
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A Standardized High-Throughput Serum Bactericidal Assay for N. gonorrhoeae published by Pel-Freez and FDA ResearchersFDA CBER researchers and Pel-Freez developed and validated a standardized, high-throughput serum bactericidal assay for Neisseria gonorrhoeae using IgG/IgM-depleted human complement. A summary of...
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You asked, we answered: Lyophilized human complement is here!Pooled and single donor human complement, native or antibody-depleted, now in a freeze-dried format: ships on cool packs, stores at −20 °C, and reconstitutes...
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Evaluate vaccine immunogenicity with OPKAOpsonophagocytic killing assays (OPKA/OPA) and multiplex OPKA (MOPA) evaluate the ability of antibodies to opsonize bacteria, facilitating their destruction by immune cells. Unlike ELISAs,...
