Custom service
Custom antibody
and antiserum production
Advance your research with U.S.-based custom antibody production and antiserum services backed by decades of rabbit immunization and production expertise.
Our products meet uncompromising quality and ethical standards.
Hear from our community
“For over a decade Pel-Freez Biologicals has been a consistent supplier of high-quality reagents and an excellent partner.”
“We have ordered from Pel-Freez for over a decade and they are always willing to go above and beyond in making sure we can obtain the right product for our specific research needs.”
Custom antibody services you can rely on
We produce high-quality rabbit custom polyclonal antibodies and antiserum through controlled immunization protocols to provide the reliability and flexibility your research depends on.
Choose our standard polyclonal antiserum service with full QC traceability, or add optional downstream services, including ELISA testing, IgG purification, B cell/mAb isolation, and more.
Standard polyclonal antiserum schedule
Our standard 9-week rabbit immunization schedule ensures consistent custom antibody and antiserum production and predictable project timelines. Each phase, from acclimation to final collection, is carefully monitored by experienced staff and overseen by our Attending Veterinarian. If your study has specific requirements, we can easily adjust our protocols to fit your research objectives.
| Week | Day | Treatment Description |
|---|---|---|
| 0 | -6+ | Acclimation/Quarantine |
| 1 | 1 |
1) Pre-immunization blood collection
2) Primary Immunization
|
| 2 | 7 | Idle – health check only |
| 3 | 14 | Idle – health check only |
| 4 | 21 |
1) Primary immunization blood collection
2) Booster #1
|
| 5 | 28 | Idle – health check only |
| 6 | 35 | Idle – health check only |
| 7 | 42 |
1) Booster #1 blood collection
2) Booster #2
|
| 8 | 49 | Idle – health check only |
| 9 | 56 | Exsanguination |
Process Overview
Discuss your
project requirements
Outline your research goals with our team to create a custom antibody production plan.
Send us your
antigen
Ship your antigen using our simple submission process for secure, tracked handling.
Production &
quality testing
Immunization and QC testing are performed under strict IACUC and USDA oversight.
Delivery to
your lab
Receive your antibodies with full QC documentation and shipment traceability.
Custom antibody and antiserum service outputs
The primary output of our custom antibody and antiserum production is high-quality terminal antiserum. Terminal antiserum is collected at the end of the immunization schedule, dispensed to your specifications, and frozen at –80 °C for long-term stability.
Intermittent serum samples, whole blood for PBMC isolation, and additional services, such as ELISA or antibody purification, can be arranged upon request.
Frequently Asked Questions
What is your standard protocol for custom polyclonal antibodies and antiserum?
Our standard protocol uses New Zealand White rabbits, 10 weeks old at immunization. Depending on project requirements, either male, female, or both may be used.
The standard 9-week schedule includes acclimation, a primary immunization, two boosters, and a final bleed for terminal antiserum collection. The client provides the antigens, with adjuvants and positive or negative controls available upon request.
Do you offer antigen design or purification?
We do not currently offer antigen design or purification services. However, we’re actively exploring in-house capabilities and welcome collaboration to better understand your needs for future development.
Can you modify the standard immunization schedule?
Yes. Our standard 9-week schedule is designed for consistency, but we can adapt it to meet your study requirements. Adjustments to booster timing, sample collection, or additional test points can be made to support your research objectives.
Do you provide ELISA results with all projects?
ELISA testing is an additional service that can be added to your project to track antibody response throughout immunization. This allows for data-driven decisions on booster timing or antibody yield and can be paired with antibody purification or other downstream services.
How is animal welfare monitored during custom antibody and antiserum production?
All projects are conducted under IACUC oversight, with our Attending Veterinarian supervising every phase of the protocol. Our animal facilities are USDA-licensed, APHIS-inspected, and FACTA-certified to ensure humane care and consistent, high-quality results. Find out more about our commitment to animal welfare here.
Should I store antibodies at –20 °C or at –80 °C? Should I aliquot them on delivery?
Either –20 °C or –80 °C is acceptable, depending on the usage, but avoid multiple freeze-thaw cycles. Our antibodies contain glycerol, which will inhibit freezing at –20 °C, but the materials will freeze at –80 °C. If the antibody will be consumed within a few weeks of delivery, we recommend storage at –20 °C. For long-term storage, we recommend subaliquoting in single-use aliquots and then storing at –80 °C.
Featured Products
Have specific antibody or antiserum needs? We can help.
Discuss your needs with our team, and we can adapt to meet your study’s requirements.
