Quickly quantify complement activity in donor samples or assess real-time stability of key sera lots using our validated CH50 testing service.
Our products meet uncompromising quality and ethical standards.
Hear from our community
“We have ordered from Pel-Freez for over a decade and they are always willing to go above and beyond in making sure we can obtain the right product for our specific research needs.”
“Pel-Freez's IgG/IgM depleted human complement has been crucial for complement-dependent bactericidal assays in my lab. The material is of the highest quality, with minimal lot-to-lot variation. This reagent has saved us considerable time and expense.”
“For over a decade Pel-Freez Biologicals has been a consistent supplier of high-quality reagents and an excellent partner.”
“Pel-Freez is our first choice as a supplier for biological matrices and complement. Their product quality and consistency of product lot to lot enables us to develop premier bacterial assays in support of the worlds’ premier vaccine biopharmaceutical companies with confidence.”
Complement activity measurement made easy
Running CH50 testing in-house is difficult. Available kits are scarce or highly perishable, and establishing a dependable hemolytic workflow requires a significant financial and time investment. These challenges make routine complement activity measurement impractical for many programs.
Our CH50 testing service gives your team access to the same validated workflow we use every day for our complement products. Using Pel-Freez’s validated in-house complement assay, we’ve optimized the classical hemolytic method with full quantitation and pooled serum reference controls on every plate. The CH50 value of a serum sample or a serum pool is valuable when screening donors or pooled serum lots for use in high throughput vaccine studies.
Support regulatory submissions with lot-specific CH50 testing
For long-term studies built on large lots of antibody-depleted complement, our CH50 testing service enables you to generate real-time stability data on the same lots used in your trials. Tracking complement activity over time on your actual study material, rather than on unrelated lots, provides more relevant stability insight to support regulatory submissions.
Process Overview
Discuss your
project
requirements
Outline your goals so our team can tailor the CH50 testing to your program needs.
Send us your
samples
We manage all sample inventory in accordance with our customer property handling ISO standards.
CH50 testing
Our validated assay tests your samples for functional complement activity.
Quality control
Our in-house quality systems personnel verify the validity of the results before they’re sent to you.
Frequently Asked Questions
What is CH50 testing used for?
CH50 testing measures total functional complement activity through the classical pathway. It is commonly used to evaluate donor serum for complement-dependent assays such as rSBA and hSBA and to support assay consistency across study samples. CH50 can also be used as an add-on stability test to monitor complement activity over time on the same antibody-depleted complement lots used in long-term clinical studies, providing lot-specific stability data that supports regulatory submissions.
What samples are required for CH50 complement assays?
CH50 assays require frozen animal or human serum samples. Each sample is evaluated using an optimized hemolytic workflow and tested alongside pooled animal or human serum controls to determine functional complement activity. Pel-Freez supplies all other required biological materials for the assay, including reference control sera and the fresh sheep red blood cells needed for the hemolytic readout.
What is the turnaround time for CH50 test results?
Turnaround time depends on project size and sample volume, but results are typically delivered after completion of testing and QC review. Pel-Freez Quality Systems verify all data before release.
Is Pel-Freez CLIA-certified?
No, Pel-Freez is not CLIA-certified, and our CH50 testing service is not for diagnostic use. We provide CH50 lab tests of human specimens for development and clinical research purposes only.
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Discuss your program’s specific requirements with our team, and we can create a customized CH50 testing service tailored to your needs.
